Analysis of EphA2 expression in a variety of pancreatic cancer cell lines, including MIA PaCa-2, PANC-1, BxPC-3, and AsPC-1 cells (ATCC) and normal immortal LT2 cells (Millipore) was performed using western blotting experiments probed with a primary antibody for the EphA2 receptor (Cell Signaling, used at 1:1,000 dilution). Here, EPHA2 is linked to familial pancreatic carcinoma.