Further, in PKM2-overexpressing PLC5 and Hep3B cells, we failed to find active apoptotic markers, such as caspase-3 and PARP-1, compared to parental cells, suggesting that PKM2 plays a role in the effect of sorafenib on HCC cell death (Figure 3C). Here, PARP1 is linked to hepatocellular carcinoma.