As metformin can modulate both glucose and fatty acid metabolism, which are key to the formation of the phospholipid precursor diacylglycerol, we have examined the effect of metformin on the rate of accumulation of PtdCho in breast cancer cells and the activities of Key enzymes involved in the formation (CK and CCT) and breakdown of PtdCho (PtdCho-PLC). This evidence concerns the gene CHKA and breast carcinoma.