In previous studies with fourth-generation cTn assays performed after stress testing,the results remained undetectable, below the CV limit of 10%, or not associated withischemia induction.25-29 Another study usinghigh-sensitivity cTnI (hscTnI), however, has found changes proportional to theintensity of the ischemia (mild and moderate-to-severe) estimated on myocardialperfusion imaging, when the sample was collected 2 and 4 hours after the stresstest. This evidence concerns the gene TNNI3 and ischemia.