BRCA2 and lung cancer: BRCA2 mRNA downregulation was confirmed by qPCR to ensure that lack of sensitization was not due to inadequate transfection (Figure 3B), and the level of BRCA2 mRNA knockdown was similar to that observed in A549 lung cancer cells (which are sensitized to olabarib by BRCA2 ASO treatment) (Figure 3C).