We used two different well-characterized cell lines, microsatellite instability MSI+ (HCT116) and MSI− (SW480) cells, as well as cells that are WT for p53 (HCT116) and mutant (SW480) [57], since 5-Fluorouracil (5FU)-mediated resistance is tightly linked to MSI and p53 status in CRC, derived from colorectal cancer cells, and we evaluated the effects of resveratrol on Sirt1 targeting using Sirt1-ASO on NF-κB signaling pathways in human CRC cells. The gene discussed is SIRT1; the disease is colorectal cancer.