Likewise, withaferin-A-induced decrease in cell viability in leukemia cells was mediated through the externalization of phosphatidylserine, a time-dependent increase in Bax/Bcl-2 ratio, the loss of mitochondrial transmembrane potential, cytochrome c release, activation of caspases-9 and -3 activation, and arresting of cells in sub-G0 phase [77]. This evidence concerns the gene CYCS and leukemia.