The best cut-off determined by the ROC curves between the fertile group and the infertile group was 0.3449 × 10−1 μg/μL, showing that the anti-ACTL7a antibodies in serum with a concentration over 0.3449 × 10−1 μg/μL were considered positive according to the ELISA. Here, ACTL7A is linked to Infertility.