The average concentration of anti-ACTL7a antibodies in the six asthenozoospermia subjects was 1.4800 ± 0.2332 μg/μL, which was much higher than for the fertile males, and all six of the asthenozoospermia sera (100%) were positive and five (83.33%) were strongly positive. Here, ACTL7A is linked to Reduced sperm motility.