To elucidate the signalling pathway involved in profilin-1-mediated cardiomyocyte-specific remodelling, we evaluated the activity of two major transcription factors (NFAT and MEF2) that govern the stress response during cardiac hypertrophy.37 Activity was measured using luciferase assays with the regulator of calcineurin (RCAN, an upstream regulator of NFAT) and MEF2 reporters. Here, PFN1 is linked to cardiac hypertrophy.