We found that compared with negative group, 10 μg of pSilencer3.1-H1-neo-Bit1-shRNA significantly suppressed tumor growth (P < 0.05) (Fig. 5a and b), but 5 μg of pSilencer3.1-H1-neo-Bit1-shRNA didn’t inhibit tumor growth (P > 0.05) (Fig. 5a and b), suggesting Bit1-shRNA mediated inhibitory effect on tumor growth displays in a dose-dependent manner. Here, PTRH2 is linked to neoplasm.