As expected, we observed that TGF-β1 treatment was able to protect HCT116 (chemosensitive) cells to the action of 5FU (Figure 7); in order to further demonstrate that the protection against 5FU is induced in chemoresistant cells exposed to 5FU, we treated chemoresistant colon carcinoma cell lines (HT-29 and HCT116p53KO) with 5FU and used such conditioned medium to protect HCT116 chemosensitive cells from 5FU-induced toxicity. This evidence concerns the gene TGFB1 and colon carcinoma.