Since it has been reported a relationship between GSK3 and TGF-β pathways in tumor progression of different carcinomas [20, 21] and it has been demonstrated the relationship between chemoresistance and TGF-β in colon cancer [17], we investigated how the TGF-β pathway could contribute to the chemoresistance/chemoreversion phenomenon in our model. This evidence concerns the gene TGFB1 and malignant colon neoplasm.