ISYNA1 and Lysosomal disease: We extended our analysis for three potential Ino1 binding proteins: GpmA, a phosphoglycerate mutase that catalyzes the production of 2,3-bisphospho-d-glycerate (2,3BPG), which was found to accumulate in ino1− cells starved of inositol (30); PefB, a penta-EF hand domain-containing protein linked to neurodegenerative and lysosomal diseases (43, 44); and Q54IX5, an uncharacterized protein with three Sel1-like repeats which was present in all three independent immunoprecipitation experiments (Fig. 8C and D).