The ampullary adenocarcinoma cell lines MDA-AMP7, AVC1, RCB1280, SNU478 and SNU869 were grown in standard culture medium and expression of KRT7, KRT20, CDX2, E-Cadherin and ZEB1 mRNA was measured by real-time PCR. The gene discussed is CDH1; the disease is ampulla of vater adenocarcinoma.