NUCB2 and Insulin resistance: Although blood glucose levels were not different between Nesf/NUCB2-Tg mice and their non-Tg littermates fed 45% HFD, serum IRI level was significantly higher in Nesf/NUCB2-Tg mice than in their non-Tg littermates (Figure 1), indicating the development of insulin resistance in Nesf/NUCB2-Tg mice fed 45% HFD.