Initially, multiplex CRISPR/Cas9 genome engineering was used to generate unspecific large deletions within the 3′UTR of the FIH1 gene in NSCLC lung cancer cells.326 In this case, cells were simultaneously cotransfected with a Cas9‐expressing plasmid and twelve sgRNAs targeting various positions in the FIH1 3′UTR. This evidence concerns the gene PTH and lung cancer.