In this work, we report a supramolecular nanocluster composed of phenylboronic acid modified β-cyclodextrin (PBCD) and adamantane modified polyethylenimine (PEI-Ada), which could load insulin and condense pCMV3-C-GFPSpark-Ins (pCMV-Ins) plasmid DNA that containing full length clone DNA of homo sapiens insulin at the same time for potential diabetes treatment (Fig. 1). This evidence concerns the gene INS and diabetes mellitus.