ADHFE1 and glioblastoma: Since an increase in HOT enzyme activity could lead to an accumulation of D-2HG, with tumorigenic effects similar to those observed in tumors with mutant IDH1/2 and 2HG has recently been shown to accumulate at high concentrations in IDH WT tumors, coupled with the fact that only a minority of GBMs carry IDH1/2 mutations, we analyzed a set of GBM samples for mutations in the HOT gene.