A detailed characterization based on Akt isoform antibodies and phosphatase experiments in neuroblastoma cells (Figs. 1 and 2) suggested that the peaks with pI 5.06, 5.14, and 5.31 correspond primarily to Akt1, whereas peaks with pI 5.42, and 5.53 correspond to mixed Akt isoforms, most likely Akt1 and Akt2 (Fig. 3). This evidence concerns the gene AKT2 and neuroblastoma.