Next, to further assess the molecular mechanisms of Hh signaling in EGFR-TKI-resistant NSCLC cells, we examined GLI1, Snail, E-cadherin, and ABCG2 expression at 0, 24, and 48 h after treatment of the EGFR-TKI-resistant cell lines H1975 and A549 with SANT-1 (40 μM). The gene discussed is GLI1; the disease is non-small cell lung carcinoma.