To assess whether the enhanced Trp-1-specific CD8+T cell frequencies were accompanied by an improvement of MAA-specific CD8+T cell effector functions, we tested PBMCs from B16 tumor-challenged mice for production of cytokines upon their in vitro stimulation with the MAA peptide pool, which included peptides representing the eight CD8+T cell epitopes expressed by the AdC68-gDMelapoly vaccine. This evidence concerns the gene CD8A and neoplasm.