Accordingly, when we monitored the exposure of LLE vesicles (as detected by the addition of Av-SRho to the culture medium) on the surface of melanoma cells with reduced SNAP-23 expression, a profound inhibition of endosomal vesicle exocytosis was observed, as detected by a lower Av-SRho uptake (Fig. 6c,d; Supplementary Movie 12). This evidence concerns the gene SNAP23 and melanoma.