Interestingly, in spite of heterogeneity between various primary cultures, experiments conducted with another primary culture GBM (G-16) ascertained that MMP-9 and MMP-2 activity levels were diminished by the mTOR inhibitors (Supplementary Fig. S5a–c), thus underscoring the effectiveness of the mTOR inhibitors for controlling invasion in GBM cells. This evidence concerns the gene MMP9 and glioblastoma.