The levels of expression of the bona fide colon cancer WNT-TCF activity biomarker AXIN2, as well as the WNT-regulated stem cell markers LGR5 and ASCL2 (e.g. [2]), were determined by RT-qPCR in transduced cells in vitro and in subcutaneous xenografts (Fig 1A). This evidence concerns the gene ASCL2 and colonic neoplasm.