RNF11 and viral infectious disease: To determine if RNF11 mRNA is indeed repressed by miR-19b-3p in the context of virus infection, a dual-luciferase reporter plasmid containing a putative binding site for miR-19b-3p and a mutant construct harboring the miR-19b-3p seed region with a 4-bp mutation were generated (Fig. 4B).