NOS3 and focal segmental glomerulosclerosis: While such investigations are beyond the scope of this methodological paper, the fact that eNOS -/- mice at this age show normal serum creatinine levels, kidney weight, and urine albumin excretion [14], suggest that the observed microCT changes may be due to poor perfusion caused by the renal lesions, including focal glomerulosclerosis and renal scaring reported in these mice [13, 14].