Multivariate analysis showed that preoperative AFP (p = 0.010), the largest tumor size >5 cm (p < 0.001), multiple tumor nodules (≤3) (p < 0.001), microscopic portal vein thrombosis (p = 0.014), and macroscopic vascular invasion (p = 0.017) were the independent prognostic factors for poor OS (Table 2). This evidence concerns the gene AFP and neoplasm.