Hence, we observed normotensive or prehypertensive premenopausal women without diabetes mellitus and normotensive or prehypertensive premenopausal women with diabetes mellitus, evaluated the levels of NO、 VEGF and GM-CSF in plasma or culture medium, and investigated the expression of tie2/Akt/eNOS signaling pathway in each groups. The gene discussed is AKT1; the disease is diabetes mellitus.