The number of tape strips required to reach a TEWL of greater than 20 g/m2/h per hour was significantly lower in the FLG-null AD group compared with all other groups (FLG-null AD: 11.25 ± 2 strips vs WT AD: 14.7 ± 4.9 strips vs FLG-null non-AD: 17.00 ± 3.9 strips vs WT non-AD: 18.9 ± 5.9 strips, P = .0001; Fig 3, B). Here, FLG is linked to Alzheimer disease.