In order to examine the efficacy of the small molecule inhibitors 13-197 (NF-kB/mTOR dual inhibitor), BI2536 (PLK1 inhibitor) and vismodegib (hedgehog inhibitor) on the proliferation and survival of neuroblastoma cells in vitro, three non-MYCN amplified (SH-SY-5Y, SK-N-SH and SK-N-AS) and three MYCN amplified (IMR-32, SK-N-BE(2) and SK-N-DZ) neuroblastoma cell lines were used. This evidence concerns the gene NFKB1 and neuroblastoma.