To verify the roles of these signaling pathways in the regulation of VEGF expression in gastric cancer cells, we performed western blot analyses and further evaluated CRMP4 expression after treating both SGC7901/HGC27 cell lines with exogenous VEGF and two independent inhibitors of the MAPK (PD98059, inhibits ERK activity) and PI3K (LY294002, inhibits AKT activity) signaling pathways. The gene discussed is AKT1; the disease is gastric cancer.