Then, we cloned putative miR-92b binding sites from ITGAV 3′UTR into the reporter plasmid and subsequent dual-luciferase reporter assay demonstrated that miR-92b could reduce luciferase activity significantly in ESCC cells; whereas mutation of the binding sites restored the luciferase activity in these transfected cells (Figure 3E and Supplementary Figure S5E). Here, ITGAV is linked to esophageal squamous cell carcinoma.