As demonstrated, KU-0060648 treatment significantly inhibited activation of PI3K (p85 phosphorylation), AKT (Ser-473 and Thr-308 phosphorylations) and mTOR (p70S6K1 Thr-389 phosphorylation) in both established (HepG2/Huh-7 lines) (Figure 4A and 4B) and primary human HCC cells (line-1, Figure 4C). Here, RPS6KB1 is linked to hepatocellular carcinoma.