Most of EGFR occured in the cell membrane in normal cells, part of EGFR internalized into the cytoplasm upon low concentration EGF (100ng/ml) stimulation, most of EGFR internalized into the cytoplasm upon high concentration EGF(10ug/ml) stimulation, both TGEV particles and EGFR internalized into the cytoplasm upon TGEV infection (Figure S3). This evidence concerns the gene EGFR and infection.