A novel non-viral gene vector, Pluronic-PEI-DR5-TAT, was successfully constructed by cross-linking LMW-PEI with Pluronic and further coupled a multifunctional peptide DR5-TAT to form the copolymer for targeting DR5-positive cancer cells and increasing cellular uptake efficiency, which is believed to increase gene transfection efficiency and reduce toxicity to normal cells. Here, TNFRSF10B is linked to cancer.