Following tumor cell disassociation, tumor cell samples were incubated with anti-CD44, anti-CD24, and anti-lineage mixture antibodies (PE-conjugated anti-CD2, CD3, CD10, CD16, CD18, CD31, and CD 140b), and then labeled by Aldefluor assay, and analyzed using MoFlo Astrios flow cytometry. The gene discussed is CD2; the disease is neoplasm.