Since the Teff/Treg ratio has been reported to be an independent prognostic factor for patients with cervical cancer, we first evaluated the numbers of intralesional Tregs (defined as CD45+CD3+CD4+CD8−CD25hiCD127lo) and found that the TME of subcutaneous tumours abounded in Tregs as compared with other tumour locations, although their numbers dramatically dropped upon vaccination30. This evidence concerns the gene CD4 and neoplasm.