CD4 and cervical cancer: Since the Teff/Treg ratio has been reported to be an independent prognostic factor for patients with cervical cancer, we first evaluated the numbers of intralesional Tregs (defined as CD45+CD3+CD4+CD8−CD25hiCD127lo) and found that the TME of subcutaneous tumours abounded in Tregs as compared with other tumour locations, although their numbers dramatically dropped upon vaccination30.