Notably, we did not observe any significant difference of Notch1 and Notch2 and its downstream genes (ASCL1, HES1, HEY1, and SLUG) mRNA level, except trace levels difference of Notch3 and Notch4 RNA level in NSCLC cells (S4 and S5 Tables). The gene discussed is ASCL1; the disease is non-small cell lung carcinoma.