This has been confirmed by Neyrinck and Lanthier [26, 27] and is consistent with our results that gene expression of macrophages markers in liver, CD68, F4/80 and CD11c, were elevated in HFD feeding while significantly reversed by NF-κB silence, indicating that inhibition of macrophages in liver, maybe largely Kupffer cells, exerted ameliorating obesity and related metabolic disorders effects in HFD-fed mice. The gene discussed is NFKB1; the disease is metabolic disease.