TNF and amyotrophic lateral sclerosis: In order to determine the effect of ALS, in the production of pro-inflammatory cytokines in MΦs, expression of IL-12 (Fig. 3A), IL-1β (Fig. 3B), TNF-α (Fig. 3C), ΙL−6 (Fig. 3D), ΤGF−β (Fig. 3E), and IL-10 (Fig. 3F) were checked.