In our study we observed that inhibition of only cathepsin D using pepstatin A did show some degree of inhibition in phago-lysosome fusion in ALS pre-treated MΦs, whereas inhibition of V-ATPase by bafilomycin A1 significantly hampered the process (Fig. 7A) (P < 0.05). This evidence concerns the gene CTSD and amyotrophic lateral sclerosis.