Visual assessment (VA) at a glance is now used to evaluate Ki67 LI in a considerable number of pathological institutions and laboratories, but there still are some limitations, for instance, the reproducibility of intermediate Ki67 LI and Ki67 LI in the moderately differentiated (G2) breast cancers in which Ki67 LI are crucial for making clinical decisions is relatively poor[6–10]. This evidence concerns the gene MKI67 and breast cancer.