PKM and breast cancer: In order to exam whether PKM2 affected the stability of the TTP protein, we overexpressed HA-tagged PKM2 or vector control in Flag-tagged TTP-overexpressing cells, and then detected exogenous TTP protein, which was decreased dramatically both in HEK293T (Fig. 3A) and breast cancer cell lines MCF7 and MDA-MB-231 (Fig. 3C,D).