By analysis of the echo intensity change of B-mode image after HIFU thermal ablation, the volume of necrotic tissues, macroscopic and microscopic examinations of necrotic tissue and immunohistochemical examination of PCNA of tumor cells, these results showed that lipid NBs had the same effect as SonoVue MBs for synergistic HIFU thermal ablation. The gene discussed is PCNA; the disease is neoplasm.