Stratum Lucidum (SL). The average number of synaptophysin-positive puncta (Dataset 3) in the SL (Figure 3A andSupplemental Figure S1) was analyzed using a 3×3 univariate ANOVA (age (PND16, PND18, PND20) and training history (HCC, ONL, MWM) as the fixed factors). The gene discussed is SYP; the disease is hepatocellular carcinoma.