Recently, a number of endo-lysosomal proteins were identified at increased levels in CSF from patients with AD including LAMP2, Lysosome-associated membrane glycoprotein 1 (LAMP1), Ras related protein Rab-3 (Rab3), Ras related protein Rab-7 (Rab7), Early endosome antigen 1 (EEA1) and pro-Cathepsin L. This was accomplished using Western blotting [28]. This evidence concerns the gene EEA1 and Alzheimer disease.