Nitroxyl donors, such as 1-nitrosocyclohexyl acetate, may have therapeutic benefits in heart failure due to the direct action of nitroxyl on myofilaments, sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2a) and ryanodine receptor 2 (RyR2) [32], which all have overall positive inotropic and lusitropic effects on the heart. The gene discussed is RYR2; the disease is heart failure.