In the present studies, we used a radiolabeled ligand (1251-Acid glycoprotein or orosomucoid (ASOR)), we found that ASGPR internalization rates with cargo in AFLD are 50 % of the control (Fig. 5a), in contrast to NAFLD model in which endocytosis rates are unchanged between control and HF-fed rats. The gene discussed is ASGR1; the disease is metabolic dysfunction-associated steatotic liver disease.