ACTA1 and metabolic dysfunction-associated steatohepatitis: To further investigate the identity of these cells, dual‐colour immunofluorescent staining for TWEAK (green) and α‐SMA (red) was performed in tissue samples from patients with NASH (Figure 3B), in addition to detection of the HSC and myofibroblast marker, vimentin (red) and TWEAK (green), in NASH and ALF samples (Figure 3C).