To further validate the autophagy induced by RBM5 overexpression, we assessed the expression levels of RBM5 and candidate markers of autophagy, LC3, LAMP1, and Beclin1, by immunohistochemistry staining in the tissue from the tumor xenografts in our in vivo studies following treatment with attenuated Salmonella carrying control plasmids or GV287-RBM5 plasmids. The gene discussed is BECN1; the disease is neoplasm.