CD4 and Chagas disease: Among patients with Chagas disease, mean levels of CD4+ T-cell counts were significantly lower in patients with reactivation (131 cells, 95% CI: -56.54–318.54) compared to patients without reactivation (322.46 cells, 95% CI: 229.45–415.47) (t-test with unequal variances: p = 0.046) (Fig 3A).