TSLP treatment for 48 h significantly promoted the percentages of Annexin V+ apoptotic cells in the culture of SW1116, SW480 and DLD-1 cells in a dose-dependent manner (Figure 3A and 3B), although higher concentrations of TSLP (100 ng/ml and 200 ng/ml) were needed to significantly promote apoptosis of colon cancer cells after 24 h incubation (Supplementary Figure S2). This evidence concerns the gene ANXA5 and colonic neoplasm.